Function Aryldialkylphosphatase

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organophosphate compounds serve substrates enzyme-catalyzed hydrolysis pte.


the enzyme has broad substrate specificity, , efficient in catalyzing reaction: pte hydrolyzes paraoxon @ rate approaching diffusion limit, indicates enzyme optimally evolved using substrate. acts on synthetic organophosphate triesters , phosphorofluoridates. not seem have natural occurring substrate , may have optimally evolved utilizing paraoxon , other common agricultural pesticides.


the products of reaction diethyl phosphoric acid , p-nitrophenol. latter product further degraded enzyme encoded 750bp downstream of opd gene, , encodes 29kda putative hydrolase may involved in degrading aromatic compounds, , works in concert pte. enzyme homologous hydrolases in pseudomonas putida, pseudomonas azelaica, rhodococcus sp., , p. fluorescens.


organophosphates not toxic bacteria, act acetylcholinesterase inhibitors in animals. species of bacteria able utilize organophosphates nutrient , carbon source.








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